Performance analysis of rapid diagnostic tests on atypical bovine
spongiform encephalopathy
John G. Gray Sandor Dudas Catherine Graham Stefanie Czub, DVM, PhD
stefanie.czub@inspection.gc.ca
Abstract
The preferred method to determine the prevalence of bovine spongiform
encephalopathy (BSE) in a country is to use immunology-based rapid-tests. Though
these tests are validated to detect C-type BSE disease–associated prion (PrPsc),
test-specific properties may influence their ability to detect H- and/or L-type
BSE PrPsc, where both are atypical from C-type PrPsc. Molecular characterization
shows atypical BSE PrPsc to have a different sensitivity to proteinase activity
and different affinities for certain prion-specific antibodies. It is important
to understand how atypical BSE PrPsc may affect the performance of rapid-tests,
which are typically dependant on the use of specific proteases and antibodies.
The current study used experimentally generated C-, H-, and L-type BSE PrPsc to
evaluate 3 tests used in various national BSE surveillance programs: an
immunochromatographic assay, a standard sandwich enzyme-linked immunosorbent
assay (stndELISA), and a PrPsc-conformation–specific ELISA (confELISA). Although
BSE PrPsc type had some effects on rapid-test performance, analytical
sensitivity for atypical BSE PrPsc on all 3 platforms was not significantly
compromised. When testing for atypical BSE PrPsc, the 3 tests were able to meet
the same requirements that the European Food Safety Authority set when
evaluating the tests for C-type BSE PrPsc.
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Saturday, August 4, 2012
Final Feed Investigation Summary - California BSE Case - July 2012
=============================================
SUMMARY REPORT CALIFORNIA BOVINE SPONGIFORM ENCEPHALOPATHY CASE
INVESTIGATION JULY 2012
Summary Report BSE 2012
Executive Summary
Saturday, August 4, 2012
Update from APHIS Regarding Release of the Final Report on the BSE
Epidemiological Investigation
in the url that follows, I have posted
SRM breaches first, as late as 2011.
then
MAD COW FEED BAN BREACHES AND TONNAGES OF MAD COW FEED IN COMMERCE up until
2007, when they ceased posting them.
then,
MAD COW SURVEILLANCE BREACHES.
Friday, May 18, 2012
Update from APHIS Regarding a Detection of Bovine Spongiform Encephalopathy
(BSE) in the United States Friday May 18, 2012
Thursday, June 21, 2012
MEATINGPLACE.COM WAVES MAGIC WAND AND EXPECTS THE USDA MAD COW FOLLIES BSE
TO BE GONE
Thursday, June 14, 2012
R-CALF USA Calls USDA Dishonest and Corrupt; Submits Fourth Request for
Extension
R-CALF United Stockgrowers of America
Friday, May 25, 2012
R-CALF USDA’s New BSE Rule Eliminates Important Protections Needed to
Prevent BSE Spread
Monday, June 18, 2012
R-CALF Submits Incomplete Comments Under Protest in Bizarre Rulemaking
“Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products”
Monday, August 6, 2012
TAFS BSE in USA August 6, 2012
BSE in USA
Monday, August 06, 2012
Atypical neuropathological sCJD-MM phenotype with abundant white matter
Kuru-type plaques sparing the cerebellar cortex
Monday, July 23, 2012
The National Prion Disease Pathology Surveillance Center July 2012
Wednesday, November 17, 2010
MAD COW TESTING FAKED IN USA BY Nebraska INSPECTOR
Senator Mike Johanns STATE Neb. inspector accused of faking mad cow
tests
Published November 17, 2010
Wednesday, December 23, 2009
Scientific Opinion on Analytical sensitivity of approved TSE rapid tests
Scientific Opinion on Analytical sensitivity of approved TSE rapid tests
Question number: EFSA-Q-2009-00687 Adopted: 10 December 2009 Summary (32 KB)
Tuesday, July 14, 2009
U.S. Emergency Bovine Spongiform Encephalopathy Response Plan Summary and
BSE Red Book Date: February 14, 2000 at 8:56 am PST
WHERE did we go wrong $$$
TSS
