Wednesday, August 8, 2012

Performance analysis of rapid diagnostic tests on atypical bovine spongiform encephalopathy

Performance analysis of rapid diagnostic tests on atypical bovine spongiform encephalopathy

John G. Gray Sandor Dudas Catherine Graham Stefanie Czub, DVM, PhD stefanie.czub@inspection.gc.ca
 
 
 
Abstract

The preferred method to determine the prevalence of bovine spongiform encephalopathy (BSE) in a country is to use immunology-based rapid-tests. Though these tests are validated to detect C-type BSE disease–associated prion (PrPsc), test-specific properties may influence their ability to detect H- and/or L-type BSE PrPsc, where both are atypical from C-type PrPsc. Molecular characterization shows atypical BSE PrPsc to have a different sensitivity to proteinase activity and different affinities for certain prion-specific antibodies. It is important to understand how atypical BSE PrPsc may affect the performance of rapid-tests, which are typically dependant on the use of specific proteases and antibodies. The current study used experimentally generated C-, H-, and L-type BSE PrPsc to evaluate 3 tests used in various national BSE surveillance programs: an immunochromatographic assay, a standard sandwich enzyme-linked immunosorbent assay (stndELISA), and a PrPsc-conformation–specific ELISA (confELISA). Although BSE PrPsc type had some effects on rapid-test performance, analytical sensitivity for atypical BSE PrPsc on all 3 platforms was not significantly compromised. When testing for atypical BSE PrPsc, the 3 tests were able to meet the same requirements that the European Food Safety Authority set when evaluating the tests for C-type BSE PrPsc.
 
 
 
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Saturday, August 4, 2012

Final Feed Investigation Summary - California BSE Case - July 2012

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SUMMARY REPORT CALIFORNIA BOVINE SPONGIFORM ENCEPHALOPATHY CASE INVESTIGATION JULY 2012

Summary Report BSE 2012

Executive Summary

Saturday, August 4, 2012

Update from APHIS Regarding Release of the Final Report on the BSE Epidemiological Investigation

in the url that follows, I have posted

SRM breaches first, as late as 2011.

then
 
 
 
MAD COW FEED BAN BREACHES AND TONNAGES OF MAD COW FEED IN COMMERCE up until 2007, when they ceased posting them.

then,
 
 
 
MAD COW SURVEILLANCE BREACHES.


Friday, May 18, 2012

Update from APHIS Regarding a Detection of Bovine Spongiform Encephalopathy (BSE) in the United States Friday May 18, 2012

Thursday, June 21, 2012

MEATINGPLACE.COM WAVES MAGIC WAND AND EXPECTS THE USDA MAD COW FOLLIES BSE TO BE GONE

Thursday, June 14, 2012

R-CALF USA Calls USDA Dishonest and Corrupt; Submits Fourth Request for Extension

R-CALF United Stockgrowers of America
 
 
 
Friday, May 25, 2012
 
 
 
R-CALF USDA’s New BSE Rule Eliminates Important Protections Needed to Prevent BSE Spread

Monday, June 18, 2012

R-CALF Submits Incomplete Comments Under Protest in Bizarre Rulemaking “Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products”

Monday, August 6, 2012

TAFS BSE in USA August 6, 2012

BSE in USA

 
 
 
 
Monday, August 06, 2012
 
 
 
Atypical neuropathological sCJD-MM phenotype with abundant white matter Kuru-type plaques sparing the cerebellar cortex

Monday, July 23, 2012

The National Prion Disease Pathology Surveillance Center July 2012

Wednesday, November 17, 2010

MAD COW TESTING FAKED IN USA BY Nebraska INSPECTOR

Senator Mike Johanns STATE Neb. inspector accused of faking mad cow tests

Published November 17, 2010

Wednesday, December 23, 2009

Scientific Opinion on Analytical sensitivity of approved TSE rapid tests

Scientific Opinion on Analytical sensitivity of approved TSE rapid tests Question number: EFSA-Q-2009-00687 Adopted: 10 December 2009 Summary (32 KB)
 
 
 
Tuesday, July 14, 2009

U.S. Emergency Bovine Spongiform Encephalopathy Response Plan Summary and BSE Red Book Date: February 14, 2000 at 8:56 am PST
 
 
 
WHERE did we go wrong $$$

TSS